Fig. 4

NDRG1 depletion causes increased levels of major lipid species. a Semi-quantitative shotgun LC MS/MS of major lipid species in SKBR3 cells. Heatmap represents fold differences in species detected in all samples standardized relative to row means. Color range 0.01–8-fold relative to row mean, N = 5 per group. See Additional file 1: Figure S5 for quantification of an independent analysis (b) in micrograms of triacylglycerol/mg protein. Individual species and sums are plotted separately (c) in nanograms of cholesterol ester per million cells. Sums of individual species and cholesterol ester (CE) species are plotted separately: N = 6 for TAG and N = 5 for CE analysis. Data represent mean +/− SD, comparisons were by the two-sided t test: **p < 0.01, ***p < 0.00001. TAG, triacylglycerols; GFP, green fluorescent protein