Fig. 2

Hepatic lipid accumulation is increased with ovariectomy (OVX) and reduced with metformin (Met). Representative images are shown for adipophilin staining in livers from lean and obese rats a prior to OVX, b after OVX-induced weight gain, and c after OVX-induced weight gain with concurrent metformin treatment. Scale bars = 500 μm. d In pre-OVX rats, adipophilin levels are not significantly different between lean and obese rats. e Metformin treatment significantly decreased hepatic adipophilin staining when compared with controls. f qMR analysis of the percent fat in livers shows that absolute lipid content was not different between lean and obese rats pre-OVX, but post-OVX hepatic lipid accumulation was significantly decreased in metformin-treated rats compared with controls (Con). *p < 0.05