Fig. 7
Identification of Net1-dependent gene signature in mammary gland tumors and analysis of human breast cancer patients. a Top 5 GO categories of genes activated in Net1 knockout, PyMT tumors. b Top 5 GO categories of genes repressed in Net1 knockout, PyMT tumors. c Signature scores in p53, PI3K, and proliferation gene expression pathways in wild-type (High Net1) or Net1 knockout (Low Net1) PyMT tumors. d Heat map of gene expression comprising Net1 signature in Net1+/+,PyMT and Net1−/−,PyMT tumors. e Frequency of Net1 gene expression signature, PI3KCA mutation or amplification, and PTEN loss of expression in human breast cancers in TCGA. f Correlation of Net1 signature with phosphatidylinositol 3-kinase catalytic subunit A (PIK3CA) overexpression or mutation, or PTEN loss, in human breast cancers. g Correlation of Net1 signature with human breast cancer subtypes. Statistical significance assessed by ANOVA. h Kaplan–Meier analysis of distant metastasis-free survival (DMFS) in human breast cancer patients with positive and negative Net1 gene expression signatures. GSE11121 analyzed. GO Gene Ontology, Lum. luminal, Net1 neuroepithelial transforming gene 1, PI3K phosphatidylinositol 3-kinase, PTEN phosphatase and tensin homolog, PyMT polyoma middle T antigen