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Fig. 6 | Breast Cancer Research

Fig. 6

From: Contributions of the RhoA guanine nucleotide exchange factor Net1 to polyoma middle T antigen-mediated mammary gland tumorigenesis and metastasis

Fig. 6

Net1 deletion reduces proliferation and angiogenesis, and increases apoptosis in a tumor cell autonomous manner. Wild-type and Net1 knockout PyMT tumor cells injected into fourth inguinal mammary gland of wild-type FVB mice. a Tumor growth. Net1+/+ = 4 mice; Net1−/− = 6 mice. b Representative examples of Ki67 staining in Net1+/+ and Net1−/− tumors. Bar = 100 μm. c Quantification of Ki67 staining in tumors. Five independent regions within each sample quantified from 4 Net1+/+ and 6 Net1−/− tumors. Bars represent median values. d Representative examples of CD31 staining (red) in Net1+/+ and Net1−/− tumors. DNA shown in blue. Bar = 20 μm. e Quantification of CD31 staining. Five independent regions from each tumor analyzed in 4 Net1+/+ and 6 Net1−/− tumors. Bars represent median values. f Representative examples of CC3 staining in Net1+/+ and Net1−/− tumors. Bar = 100 μm. g Quantification of CC3 staining. CC3 hot spots analyzed in 4 Net1+/+ and 6 Net1−/− tumors. Bars represent median values. h Representative examples of lung metastasis in mice injected with Net1+/+ and Net1−/− cells. Bar = 1000 μm. i Quantification of lung metastasis in 6 Net1+/+ and 6 Net1−/− mice. Bars represent median values. *P < 0.05; **P < 0.01; ***P < 0.001. Mets metastases, Net1 neuroepithelial transforming gene 1, PyMT polyoma middle T antigen

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