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Fig. 1 | Breast Cancer Research

Fig. 1

From: Contributions of the RhoA guanine nucleotide exchange factor Net1 to polyoma middle T antigen-mediated mammary gland tumorigenesis and metastasis

Fig. 1

Net1 deletion delays tumorigenesis and inhibits metastasis in MMTV-PyMT mice. a Kaplan–Meier analysis of tumor onset. Net1+/+,PyMT = 23 mice; Net1+/−,PyMT = 23 mice; Net1−/−,PyMT = 21 mice. b Survival analysis. Mice were euthanized when the largest tumor reached 2.5 cm. Net1+/+,PyMT = 18 mice; Net1+/−,PyMT = 14 mice; Net1−/−,PyMT = 14 mice. c Aggregate tumor mass in MMTV-PyMT mice. Net1+/+ = 22 mice; Net1+/− = 14 mice; Net1−/− = 7 mice. d Examples of lungs from Net1+/+,MMTV-PyMT and Net1−/−,MMTV-PyMT mice. Bar = 2 mm. e Examples of H&E-stained lung sections from Net1+/+,MMTV-PyMT and Net1−/−,MMTV-PyMT mice. Bar = 2 mm. f Quantification of metastatic nodules on lung surface in genotypes shown. Net1+/+,PyMT = 20 mice; Net1+/−,PyMT = 10 mice; Net1−/−,PyMT = 15 mice. g Quantification of metastases in lung sections in genotypes shown. Net1+/+,PyMT = 12 mice; Net1+/−,PyMT = 9 mice; Net1−/−,PyMT = 11 mice. h Quantification of lung area occupied by metastases in genotypes shown. Net1+/+,PyMT = 11 mice; Net1+/−,PyMT = 9 mice; Net1−/−,PyMT = 10 mice. Bars represent median values. *P < 0.05; **P < 0.01; ***P < 0.001. Met. metastasis, Mets metastases, Net1 neuroepithelial transforming gene 1, n.s. not significant, PyMT polyoma middle T antigen

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