Fig. 6

UNC5A knockdown results in elevated epidermal growth factor receptor (EGFR) and phospho-AKT. a EGFR protein levels in sh-Control and sh-UNC5A TMCF7 and T-47D cells. b Phospho-AKT and phospho-ERK levels in sh-Control and sh-UNC5A TMCF7 cells with or without estradiol (E2) or HRG-β1 (20 ng/ml) treatment. Cells were treated with vehicle, E2 for 5 and 15 min, and HRG-β1 for 15 min. c Phospho-AKT1 and phospho-AKT2 levels in sh-Control and sh-UNC5A TMCF7 cells. d Representative EGFR staining pattern in breast tumors with low or high expression (left, scale bar = 100 μm). Summary of the correlation analysis between EGFR and UNC5A from breast cancer TMA using Spearman’s correlation coefficient (right). UNC5A and EGFR expression was inversely correlated (p < 0.001, n = 179). e Protein levels of estrogen receptor (ER)α, EGFR, BCL2, and TP63 in sh-Control and sh-UNC5A TMCF7 (left) and T-47D (right) cells after treatment with ICI-182,780 (Fulvestrant)