Fig. 2

Blocking ErbB3 inhibits macrophage-induced transendothelial migration. a MDA-MB 231 cells were placed in transwells coated with Matrigel and endothelial cells, either alone (231 Alone) or in the presence of macrophages (231 + BAC), and left untreated (No Ab) or with either an IgG isotype control (IgG) or ErbB3 blocking antibody (ErbB3). Transendothelial migration was measured after 24 h. Results are shown as the mean and SEM of the number of tumor cells crossing the endothelial layer per field. N = 4; **p < 0.01, t test. b BT549 cells were placed in the transendothelial migration (iTEM) assay either alone (BT549 Alone) or with macrophages (BT549 + BAC) either untreated, with a control IgG, or with an ErbB3 blocking antibody. Results are shown as the mean and SEM of the number of tumor cells crossing the endothelial layer per field. N = 3; *p < 0.05, t test