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Fig. 5 | Breast Cancer Research

Fig. 5

From: IMP1 regulates UCA1-mediated cell invasion through facilitating UCA1 decay and decreasing the sponge effect of UCA1 for miR-122-5p

Fig. 5

Urethral carcinoma-associated 1 (UCA1) functions as a miR-122-5p sponge. a Upper: antibody pulldown of Ago2 protein. Lower; UCA1 levels in the immunoprecipitates of Ago2 were measured by RT-qPCR: *P < 0.05. b RT-qPCR analysis shows the relative levels of UCA1 in MDA231 cells after ectopic expression of miR-122-5p and antisense-oligonucleotides to miR-122-5p: n.s, not significant. c Upper: the entire UCA1 sequence was cloned into the downstream region of the renilla luciferase gene of the PsiCHECK 2 dual luciferase reporter construct (denoted as pluc-UCA1). Lower: 293 cells were transfected with pluc-UCA1 (NC), or pluc-UCA1 in combination with the UCA1-siRNA or UCA1 vector. Luciferase activity was determined by luciferase reporter assays. Activity of Renilla luciferase was normalized to the activities of firefly luciferase: **P < 0.01 as determined by Student’s t test. d and e The 293 T and MDA231 cells were transfected with pluc-UCA1 reporter (NC) or co-transfected with mature miR-122-5p mimics in combination with pluc-UCA1 reporter or the reporter harboring mutated miR-122-5p binding site (pluc-UCA1m). Relative luciferase activity is presented as means ± SD from three independent experiments: **P < 0.01; n.s, not significant; WT, wild-type

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