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Fig. 7 | Breast Cancer Research

Fig. 7

From: Isocitrate dehydrogenase 1–snail axis dysfunction significantly correlates with breast cancer prognosis and regulates cell invasion ability

Fig. 7

Depletion of isocitrate dehydrogenase 1 (IDH1) reduces the level of intracellular α-KG to stabilize hypoxia-inducible factor-1 alpha (HIF1α). a The effect of IDH1 depletion on HIF1α mRNA expression was analyzed through quantitative RT-PCR; *p < 0.05; N.C, scramble control. b, c The protein stability of HIF1α was examined in IDH1-depleted cells and control cells in the presence of 20 μg/mL of cycloheximide (CHX). Cells treated with CHX for the indicated time points were harvested for western blotting analysis; *p < 0.05. d Analysis of the intracellular level of α-KG in the control and IDH1-depleted cells using the UPLC system coupled with QT of MS. *** p < 0.001. e The effect of 1 mM α-KG treatment on HIF1α protein expression was analyzed through western blotting. f The intracellular level of α-KG in cells with the indicated treatments is shown; *p < 0.05, ***p < 0.001. Each experiment was performed in triplicate

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