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Fig. 3 | Breast Cancer Research

Fig. 3

From: Isocitrate dehydrogenase 1–snail axis dysfunction significantly correlates with breast cancer prognosis and regulates cell invasion ability

Fig. 3

miR-32-5p and miR-92b-3p suppressed isocitrate dehydrogenase (IDH1) expression by directly targeting its 3′-UTR in breast cancer cells. a The 15 putative miRNA candidates that were predicted for targeting the 3′-UTR of IDH1 using TargetScan and MicroRNA.org. TCGA, The Cancer Genome Atlas. b The expression levels of the predicted candidates in 778 breast cancer tissues and 87 adjacent normal tissues were obtained from TCGA database. c and d After transfecting MDA-MB-231 cells with miR-32-5p and miR-92b-3p mimics, the individual expression levels were examined using real-time PCR. N.C, scramble control. e and f The ectopic expression of miR-32-5p and miR-92b-3p suppressed the endogenous expression of mRNA and proteins. g and h Schema of the luciferase constructs. The miR-32-5p and miR-92b-3p targeting sequence in 3’UTR sequence of IDH1 is shown in the upper panel and the mutant (mut) of its seed region is shown in red, respectively. i and j Schema of the luciferase reporter constructs (upper panels). The relative luciferase activity of the reporter with wild-type and mutant 3′-UTR of IDH1 was determined in breast cancer cells co-transfected with miR-32-5p and miR-92b-3p mimic, respectively. The firefly luciferase activity was used as the normalization control

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