Fig. 4

Identification of reproducible broad-spectrum phosphatase and tensin homolog-synthetic sick/lethal (PTEN-SSL) genes. a Cmparison of the secondary short hairpin RNA (shRNA) screen described in this study and three independent RNA interference (RNAi) screening datasets selected for reanalysis: Breast Functional Genomics [17], Cancer Dependency Map [19], and Kinase Dependency Profiles [18]. b The p values from the one-sided Wilcoxon rank sum test for whether PTEN- cell lines were more sensitive (lower ATARiS scores) to knockdown of each gene compared to PTEN+ cell lines. Each bar represents one of 727 genes in the secondary screen that had ATARiS gene solutions. Possible broad-spectrum PTEN-SSL hits were selected using the same criterion (p < 0.05, uncorrected for multiple testing) for each screen and are shown as red bars. PIK3CB, NUAK1, STK11, ADAMTS20, AP1M2, and HMMR were found to be hits in our reanalysis of ≥ 1 of the previous screens. c Number of overlaps in possible broad-spectrum PTEN-SSL genes that were identified by this study and in reanalysis of three previous screens; p values based on one-sided hypergeometric distribution