Skip to main content
Fig. 1 | Breast Cancer Research

Fig. 1

From: Affinity proteomic profiling of plasma for proteins associated to area-based mammographic breast density

Fig. 1

Study overview. a Samples comprised plasma from women with high and low absolute volumetric breast density (High VD and Low VD) matched on age and body mass index (BMI) from the population-based KARMA cohort (Sample Set 1, N = 729; Sample Set 2, N = 600). In Sample Set 1, an additional set of 139 individuals (Karma Normal) was included. For the experimental procedure, two antibody suspension bead arrays (SBA1 and SBA2) were created with antibodies available from the Human Protein Atlas: 249 and 196 proteins were targeted. These proteins were selected from breast-cancer-related literature and proteins annotated to extracellular matrix. Both bead arrays were used for the screening of each plasma sample set (Assay 1–4). b The plasma protein profiles that were generated in the four assays were annotated and filtered based on technical quality assessments. Association with absolute area-based breast density (AD) was then assessed by quantile regression analysis, adjusting for age and BMI. Combining the results from regression analyses performed within each sample set by meta-analysis resulted in candidate protein profiles with linear associations to AD

Back to article page
\