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Fig. 6 | Breast Cancer Research

Fig. 6

From: Integrin-uPAR signaling leads to FRA-1 phosphorylation and enhanced breast cancer invasion

Fig. 6

Phosphorylation of Fos-related antigen 1 (FRA-1) is required for transcriptional activity. a Immunoblots from BRC-31 cells transfected with siRNA to plaur and fosl1 (UPAR KD/fFRA-1 KD) or scrambled control (Scrambled) were subsequently transfected with either empty vector (VC), FRA-1 wild-type (WT), FRA-1-S252AS265A (S252AS265A) or FRA-1-S252DS265D (S252DS265D) expression vectors. b Gene expression analysis of FRA-1 regulated transcriptional targets, chloride channel accessory 2 (clca2), adhesion G protein-coupled receptor G6 (adgrg6), C-X-C motif chemokine ligand 8 (cxcl8) and C-C motif chemokine ligand 5 (ccl5). Expression values are normalized to the scrambled control. Error bars represent the standard error of the mean. *P < 0.02

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