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Fig. 1 | Breast Cancer Research

Fig. 1

From: Phosphoproteomics reveals network rewiring to a pro-adhesion state in annexin-1-deficient mammary epithelial cells

Fig. 1

Phosphoproteome analysis of annexin-1 (ANXA1)-responsive phosphorylation changes. a Schematic representation of the phosphoproteome workflow used for the analysis of ANXA1+/- and ANXA1-/- mammary gland epithelial cells. The cells were stable isotope labeling with amino acids in cell culture (SILAC)-adapted and a total of four biological replicates including label reverse experiments were performed. b Correlation between the forward (Fwd) and reverse (Rev) experiments. Positive correlation between the forward and negative correlation between the label swap experiments can be observed. c Distribution of quantified phosphopeptides in one representative replicate. Those sites that were reliably regulated above or below the MAD threshold of ± 2 in three out of four replicates were considered ANXA1-responsive. The top 50 regulated sites with increased and decreased phosphorylation are shown. The robust z score is based on log2 normalized fold changes. FASP, filter-aided sample preparation; LC-MS/MS, liquid chromatography-tandem mass spectrometry; SAX, strong ion exchange

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