Fig. 5

Estrogen receptor (ER)α co-localization with proliferation markers is enhanced in obese tissue. a Proliferating cells were quantified in mammary epithelial cells in tissue from adult C57Bl/6 mice fed a control diet (Con) or high fat diet (HFD) using immunofluorescence for 5-bromo-2′-deoxyuridine (BrdU) in the mammary glands (n = 7 mice/group). Five images were quantified per × 400 field/mouse. b Immunofluorescence for Ki67 expression in breast epithelial cells from reduction mammoplasty tissue. Correlation scatterplot with best fit line demonstrating the average percentage of Ki67-positive cells per × 400 field, against body mass index (BMI). Three images/lobule were analyzed for each sample (n = 28). c Immunofluorescence for BrdU and ERα in mammary glands from mice fed Con or HFD (n = 7 mice/group). Five images were quantified per × 400 field/mouse. d Immunofluorescence for Ki67 and ERα in breast epithelial cells from reduction mammoplasty tissue. Correlation scatterplot with best fit line depicting the percentage of cells that co-label with antibodies for Ki67 and ERα per × 400 field with respect to BMI. Three images/lobule were analyzed for each sample (n = 27). Magnification bar = 100 μm