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Fig. 1 | Breast Cancer Research

Fig. 1

From: Nanopore sequencing of full-length BRCA1 mRNA transcripts reveals co-occurrence of known exon skipping events

Fig. 1

Example of fragments obtained from different long-range PCR assays from a LCL. Three different protocols were used to generate a pool of PCR amplicons for nanopore sequencing: a Protocol 1, b Protocol 2 and c Protocol 3. Primer annealing temperature indicated above each lane. Reference markers labelled for size in base pairs. d. Sanger sequence trace of PCR products in (a) (indicated by black triangle). e. BLAT alignment tool showing sequence match to BRCA1 using the UCSC Genome Browser

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