Fig. 3

miR-105/93-3p activates Wnt/β-catenin signaling through downregulation of SFRP1 to promote cisplatin resistance. a Bioinformatic analysis was performed with the DAVID online tool to identify potential cell signaling pathways that may be modulated by miR-105/93-3p. b miR-105/miR-93-3p were overexpressed in HCC70 or silenced in BT-549 to examine β-catenin activity using the TOP/FOP reporter assay 48 hours post transfection. c Silencing of miR-105/93-3p with or without β-catenin overexpression in BT-549 cells to determine cell viability after 20 μM cisplatin treatment. Treatments were normalized to control. d Ectopic overexpression or knockdown of miR-105 or miR-93-3p to examine the potential target genes of miR-105 and miR-93-3p by qPCR. e Ectopic overexpression of Flag-Ago2 with indicated miRNAs in 293 T cells. Flag antibody was used to precipitate miRNA-Ago2-mRNA complex. The expression of SFRP1 mRNA in complex with miR-93-3p and miR-105 was determined by PCR. PC positive control. f Overexpression or knockdown of indicated miRNAs to determine SFRP1 expression level by Western blot at 48 hours post-transfection. g Ectopic overexpression of miR-105/93-3p with or without SFRP1 in MB-231 cells to examine cell viability after 20 μM cisplatin treatment. Treatments were normalized to control. Mean ± SEM; ^* P < 0.05, ^*** P < 0.001, n.s. no significance