Fig. 1

Human bone tissue co-culture model. a Femoral head removal during total hip replacement (THR) surgery (image credit: Moran & Rowley – Visualhistology.com). b Discarded femoral head exposing trabecular bone (white arrow). c Isolated trabecular bone tissue fragments (bar = 3 mm). d Panel of breast cancer cell lines transfected with luciferase and enhanced green fluorescence protein (EGFP): estrogen receptor-positive (ER+) MCF-7, ZR-75 and T-47D, ER-/human epidermal growth factor receptor 2 (Her2) + SK-BR-3, and ER- MDA-MB-231 and MCF-10A cells. e Hematoxylin and eosin stained histologic sections of trabecular bone tissues from a subset of THR specimens illustrating mineralized and marrow compartments