Fig. 3

Increased fucosyltransferase 8 (FUT8) expression is associated with the invasive ability of breast cancer cell lines. a Cell morphology of normal human epithelial cells (MCF-10A), low-metastatic breast cancer cells (T-47D) and highly invasive breast cancer cells (MDA-MB-231 and Hs578T). Of note, on Transwell assay, the former two cell lines were non-invasive and had an epithelium-like morphology (upper), whereas the latter two were highly invasive and had a spindle-shaped, fibroblastic mesenchymal phenotype (lower). b Western blot analysis of FUT8 protein level in cells. The invasion ability (upper panel) of breast carcinoma cell lines was determined by Transwell invasion assay. Data are mean ± SD. OD, optical density