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Fig. 1 | Breast Cancer Research

Fig. 1

From: FUT8 promotes breast cancer cell invasiveness by remodeling TGF-β receptor core fucosylation

Fig. 1

Fucosyltransferase 8 (FUT8) expression is upregulated and core fucosylation is increased during epithelial-mesenchymal transition (EMT) in MCF-10A cells. a-b Transforming growth factor-β (TGF-β)-induced EMT in a 3D culture system. After TGF-β1 treatment for 8 days, TGF-β-induced EMT was characterized by morphological transformation of polarized, acini-like spheroids into a fibroblastic cell shape (a) and verified molecularly by E-cadherin downregulation and vimentin upregulation (b). c FUT8 is the only FUT gene highly upregulated during TGF-β1-stimulated EMT. Gene expression profiling data on FUT genes presented as log2-fold ratio difference between TGF-β-induced EMT and control MCF-10A cells (left panel). Quantitative PCR analysis of FUT8 mRNA expression before and after TGF-β-induced EMT (right panel). d Western blot analysis of FUT8, E-cadherin, and vimentin protein expression after TGF-β-induced EMT. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was a loading control. e Lens culinaris agglutinin immunofluorescence staining (carbohydrate probe for core fucose) of cell-surface core fucosylation during EMT. Scale bar = 50 μm. Data are mean ± SE. kDa, kiloDalton

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