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Fig. 2 | Breast Cancer Research

Fig. 2

From: Endophilin A2 promotes HER2 internalization and sensitivity to trastuzumab-based therapy in HER2-positive breast cancers

Fig. 2

Elevated surface human epidermal growth factor receptor 2 (HER2) levels upon Endo II silencing in HER2-positive human breast cancer cells. a Lysates from a panel of human breast cell lines were subjected to immunoblot with antibodies to epidermal growth factor receptor (EGFR), HER2, endothelin A2 (Endo II), and loading control α-Actinin. b Immunoblot analysis of HCC1954 cells with expression of two different shRNAs from GIPZ or pLKO lentiviral vectors resulted in stable knock-down (KD) of Endo II. Controls included cell lines expressing either a non-targeting shRNA (NT) or an empty vector (Vec) for GIPZ and pLKO models, respectively. c Cell growth curves were prepared by counting viable HCC1954 control and Endo II KD cells for both GIPZ (left) and pLKO (right) models at 24, 48 and 72 hours post plating. d Flow cytometry analysis of surface HER2 levels on HCC1954 pLKO Vec and Endo II KD1 cells measured using a HER2 affibody, and displayed as a representative histogram (left) or by the median fluorescence intensity (MFI) for three independent experiments (**P < 0.01)

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