Fig. 1

Endothilin A2 (Endo II) expression and association with poor prognosis in human epidermal growth factor receptor-2 (HER2)-positive (HER2+) breast cancer. a Representative images for immunohistochemical staining of Endo II in human breast tumors grouped by molecular subtype from a tissue microarray (TMA) with paired primary and lymph node metastases (n = 103). b, c Staining intensity was quantified using Imagescope software to generate tumor-specific H-scores for each primary tumor (b) or lymph node metastasis (c). d, e Kaplan–Meier plots for Endo II transcript levels (encoded by Sh3gl1) relative to relapse-free survival (d) (n = 146) and overall survival (e), (n = 56) are shown for patients with lymph-node positive HER2 tumors with up to 10 years of follow up. For high vs low Endo II groups, the median overall survival differences were 21 months (high Endo II) vs 63 months (low Endo II)