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Fig. 4 | Breast Cancer Research

Fig. 4

From: SPEN, a new player in primary cilia formation and cell migration in breast cancer

Fig. 4

SPEN regulates migration in ciliated breast epithelial cells. a and b Cell migration analysis of MCF10A, BT20, MDA-MB-436, and Hs578T cells treated with siRNA control or siRNA targeting SPEN as evaluated by Boyden chamber assay. Represented is the mean (±SEM) percentage migration, normalized to the control, in least three independent experiments. Representative pictures showing cell migration in MCF10A, BT20, MDA-MB-436, and Hs578T cells treated with siRNA control or siRNA for SPEN as evaluated by Boyden chamber assay. c Percentage of ciliated cells in several lines of breast epithelial cells. d qRT-PCR of SPEN expression in BT20 and MDA-MB-436 (436) cells treated with siRNA for SPEN, normalized to siRNA control-treated cells. Represented is the mean (±SEM) expression value of SPEN in three biological replicates. e Immunoprecipitated (IP) SPEN protein levels in BT20 and MDA-MB-436 (436) cells treated with siRNA control or siRNA against SPEN. Immunoprecipitation with nonspecific rabbit IgG was done as a negative control. *** P ≤ 0.005; ** P ≤ 0.01; * P ≤ 0.05. IB Immunoblot, IgG Immunoglobulin G, siRNA Small interfering RNA, SPEN Split ends

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