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Fig. 3 | Breast Cancer Research

Fig. 3

From: SPEN, a new player in primary cilia formation and cell migration in breast cancer

Fig. 3

SPEN regulates migration in T47D cells. a and b Cell migration analysis of T47D-CTL and T47D-SPEN as evaluated by Boyden chamber assay. Represented is the mean (±SEM) percentage migration, normalized to the control, in least three biological replicates. Representative pictures showing cell migration in T47D-CTL and T47D-SPEN cells as evaluated by Boyden chamber assay. c Representative immunoblot (IB) showing KIF3A protein levels in MCF10A cells treated with siRNA control or siRNA against KIF3A. d Percentage of ciliated cells in MCF10A cells treated with siRNA control or siRNA targeting KIF3A. e and f Cell migration analysis of MCF10A cells treated with siRNA control or siRNA targeting KIF3A as evaluated by Boyden chamber assay. Represented is the mean (±SEM) percentage migration, normalized to the control, in least three independent experiments. Representative pictures showing cell migration in MCF10A cells treated with siRNA control or siRNAs for KIF3A as evaluated by Boyden chamber assay. g Representative IB showing KIF3A protein levels in MDA-MB-436 cells treated with siRNA control or siRNA against KIF3A. h Relative cell number of MDA-MB-436 cells treated with siRNA control or siRNA targeting KIF3A 72 h after transfection. i and j Cell migration analysis of MDA-MB-436 cells treated with siRNA control or siRNA targeting KIF3A as evaluated by Boyden chamber assay. Represented is the mean (±SEM) percentage migration, normalized to the control, in least three independent experiments. Representative pictures showing cell migration in MDA-MB-436 cells treated with siRNA control or siRNA for KIF3A as evaluated by Boyden chamber assay. k Representative IB showing KIF3A protein levels in T47D-SPEN cells treated with siRNA control or siRNA against KIF3A. l and m Cell migration analysis of T47D-SPEN cells treated with siRNA control or siRNA targeting KIF3A as evaluated by Boyden chamber assay. Represented is the mean (±SEM) percentage migration, normalized to the control, in least three independent experiments. Representative pictures showing cell migration in T47D-SPEN cells treated with siRNA control or siRNA for KIF3A as evaluated by Boyden chamber assay. siRNA Small interfering RNA, SPEN Split ends

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