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Fig. 2 | Breast Cancer Research

Fig. 2

From: SPEN, a new player in primary cilia formation and cell migration in breast cancer

Fig. 2

SPEN regulates primary cilia formation. a qRT-PCR of SPEN expression in MCF10A treated with siRNA control or siRNA for SPEN. Represented is the mean (±SEM) expression value of SPEN in at least three biological replicates, normalized to siRNA control-treated cells. b Immunoprecipitated (IP) SPEN protein levels in MCF10A cells treated with siRNA control or siRNA against SPEN. Immunoprecipitation with nonspecific rabbit IgG was done as a negative control. c Representative images of primary cilia in MCF10A cells treated with siRNA control or siRNA against SPEN. Arrows point to primary cilia (scale bar = 5 μm). d Venn diagram showing the intersection of the list of genes up- and downregulated by SPEN knockdown in MCF10A cells and a curated list of genes involved in ciliary biology. The microarray data for this study can be found on ArrayExpress under the accession number [E-MTAB-4975]. e qRT-PCR of SPEN expression in Hs578T cells treated with siRNA control or siRNA for SPEN. Represented is the mean (±SEM) expression value of SPEN in at least three biological replicates, normalized to siRNA control-treated cells. f Immunoprecipitated SPEN protein levels in Hs578T cells treated with siRNA control or siRNA against SPEN. Immunoprecipitation with nonspecific rabbit IgG was done as a negative control. g Percentage of ciliated cells in Hs578T cells treated with siRNA control or siRNA targeting SPEN. h Fraction of ciliated cells in two lines of fibroblasts treated with siRNA for SPEN, normalized to siRNA control-treated cells. i qRT-PCR of SPEN expression in two lines of fibroblast-treated siRNA for SPEN, normalized to siRNA control-treated cells. Represented is the mean (±SEM) expression value of SPEN in three biological replicates. *** P ≤ 0.005; ** P ≤ 0.01; * P ≤ 0.05). DAPI 4′,6-Diaminodino-2-phenylindole, IgG Immunoglobulin G, siRNA Small interfering RNA, SPEN Split ends

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