Fig. 3

Observations in knockout gynecology. This figure illustrates the ovaries, uterus, and vagina of a 120-week-old 129:Stat1 ^−/−nulliparous female. a This low-magnification image shows that the right ovary is obliterated by a large cyst (green asterisk). Arrows B, C, and D indicate regions of interest for the higher-magnification images shown in b, c, and d, respectively. Scale bar = 5 mm. b The contralateral ovary is largely replaced by multiple vascular channels filled with red blood cells. Scale bar = 400 μm. c The cyst is lined with a tall columnar epithelium with apical nuclei characteristic of rete cysts of the mouse ovaries. Scale bar = 200 μm. d In spite of the destruction of the ovaries, the vaginal surface has a layer of bluish mucinous cells associated with proestrus. This indicates a functional estrous cycle. Scale bar = 100 μm