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Table 1 Primers used for real-time quantitative reverse transcription-polymerase chain reaction

From: Concurrent antitumor and bone-protective effects of everolimus in osteotropic breast cancer

Targeted gene

Primer sequences (5′-3′)

ACTB

CCAACCGCGAGAAGATGA

CCAGAGGCGTACAGGGATAG

ALP

CAACCCTGGGGAGGAGAC

GCATTGGTGTTGTACGTCTTG

OPG

GAACCCCAGAGCGAAATACAG

TAGCAGGAGACCAAAGACACTG

RUNX2

CAGATGGGACTGTGGTTACTG

TGGGGAGGATTTGTGAAGAC

OCN

TGAGAGCCCTCACACTCCTC

ACCTTTGCTGGACTCTGCAC

Actb

GATCTGGCACCACACCTTCT

GGGGTGTTGAAGGTCTCAAA

Alp

CTACTTGTGTGGCGTGAAGG

CTGGTGGCATCTCGTTATCC

Opg

CCTTGCCCTGACCACTCTTA

ACACTGGGCTGCAATACACA

Runx2

CCCAGCCACCTTTACCTACA

TATGGAGTGCTGCTGGTCTG

Ocn

GCGCTCTGTCTCTCTGACCT

ACCTTATTGCCCTCCTGCTT

Rankl

CACTGAGGAGACCACCCAAG

GAGATGAAGAGGAGCAGAACG

Oscar

TCTGCCCCCTATGTGCTATC

CTCCTGCTGTGCCAATCAC

Trap

ACTTGCGACCATTGTTAGCC

AGAGGGATCCATGAAGTTGC

Ctsk

AAGTGGTTCAGAAGATGACGGGAC

TCTTCAGAGTCAATGCCTCCGTTC

  1. Abbreviations: ACTB/Actb β-Actin, ALP/Alp Alkaline phosphatase, Ctsk Cathepsin K, OCN/Ocn Osteocalcin, OPG/Opg Osteoprotegerin, RANKL/Rankl Receptor activator of nuclear factor-κB ligand, RUNX2/Runx2 Runt-related transcription factor 2, TRAP/Trap Tartrate-resistant acid phosphatase