Table 3 Primers used in this study
Primer name | Nucleotide sequence | |
|---|---|---|
L32 | RT sequence | CAGAAAACGTGCACATGAGCTGC |
Forward sequence (outer nested) | CAGGGTTCGTAGAAGATTCAAGGG | |
Reverse sequence (outer nested) | CTTGGAGGAAACATTGTGAGCGATC | |
Forward sequence (inner nested) | GATCTTGATGCCCAACATTGGTTATG | |
Reverse sequence (inner nested) | GCACTTCCAGCTCCTTGACG | |
Carbonic Anhydrase 9 (for hypoxia validation) | Random priming used | Â |
Forward | CCTCAAGAACCCCAGAATAATGC | |
Reverse | CCTCCATAGCGCCAATGACT | |
E-cadherin | RT sequence | GTCAGCCAGCTTCTTGAAGCGATT |
Forward sequence | GCCCTGCCAATCCCGATGAAA | |
Reverse sequence | GGGGTCAGTATCAGCCGCT | |
Vimentin | RT sequence | CCGTGAGGTCAGGCTTGGAAA |
Forward sequence | GCTTCAGAGAGAGGAAGCCGAAAA | |
Reverse sequence | CCGTGAGGTCAGGCTTGGAAA | |
Endogenous E-cadherin (CDH1-3′UTR) | RT sequence | GCACTTGGGGATTCTGGGCTTT |
Forward sequence | GTGCCTAAAGTGCTGCAGCCAAA | |
Reverse sequence | GTACAAACCACGGATCTTGTGTCAGAAA | |
Exogenous E-cadherin (for 468-CDH1 construct) | RT sequence | GAAAGGACAGTGGGAGTGGCACTTT |
Forward sequence | CCTGAACTCCTCAGAGTCAGACAAA | |
Reverse sequence | GTGGCACCTTCCAGGGTCAAGGAA | |
E-cadherin shRNA (for 468-shCDH1 constructs) | RT sequence | CCAGCTCAGCCCGAGTGGAAAT |
Forward sequence | CCTCCCATCAGCTGCCCAGAAAA | |
Reverse sequence | CTCTGTCACCTTCAGCCATCCTGTTT | |
Snail 1 | RT sequence | CGCAGACAGGCCAGCTCAGGAAT |
Forward sequence | CACATCCTTCTCACTGCCATGGAATT | |
Reverse sequence | GCTGCCCTCCCTCCACAGAAAT | |
Estrogen Receptor 1 | RT sequence | CCAGGGCCACGCTGGGAAATGAA |
Forward sequence | GTTCCAGTGGGCACTGTACTTGGATCTT | |
Reverse sequence | CAGCTCCATGCCCCAGGGCTAAAT | |