|
L32
|
RT sequence
|
CAGAAAACGTGCACATGAGCTGC
|
|
Forward sequence (outer nested)
|
CAGGGTTCGTAGAAGATTCAAGGG
|
|
Reverse sequence (outer nested)
|
CTTGGAGGAAACATTGTGAGCGATC
|
|
Forward sequence (inner nested)
|
GATCTTGATGCCCAACATTGGTTATG
|
|
Reverse sequence (inner nested)
|
GCACTTCCAGCTCCTTGACG
|
|
Carbonic Anhydrase 9 (for hypoxia validation)
|
Random priming used
| |
|
Forward
|
CCTCAAGAACCCCAGAATAATGC
|
|
Reverse
|
CCTCCATAGCGCCAATGACT
|
|
E-cadherin
|
RT sequence
|
GTCAGCCAGCTTCTTGAAGCGATT
|
|
Forward sequence
|
GCCCTGCCAATCCCGATGAAA
|
|
Reverse sequence
|
GGGGTCAGTATCAGCCGCT
|
|
Vimentin
|
RT sequence
|
CCGTGAGGTCAGGCTTGGAAA
|
|
Forward sequence
|
GCTTCAGAGAGAGGAAGCCGAAAA
|
|
Reverse sequence
|
CCGTGAGGTCAGGCTTGGAAA
|
|
Endogenous E-cadherin (CDH1-3′UTR)
|
RT sequence
|
GCACTTGGGGATTCTGGGCTTT
|
|
Forward sequence
|
GTGCCTAAAGTGCTGCAGCCAAA
|
|
Reverse sequence
|
GTACAAACCACGGATCTTGTGTCAGAAA
|
|
Exogenous E-cadherin (for 468-CDH1 construct)
|
RT sequence
|
GAAAGGACAGTGGGAGTGGCACTTT
|
|
Forward sequence
|
CCTGAACTCCTCAGAGTCAGACAAA
|
|
Reverse sequence
|
GTGGCACCTTCCAGGGTCAAGGAA
|
|
E-cadherin shRNA (for 468-shCDH1 constructs)
|
RT sequence
|
CCAGCTCAGCCCGAGTGGAAAT
|
|
Forward sequence
|
CCTCCCATCAGCTGCCCAGAAAA
|
|
Reverse sequence
|
CTCTGTCACCTTCAGCCATCCTGTTT
|
|
Snail 1
|
RT sequence
|
CGCAGACAGGCCAGCTCAGGAAT
|
|
Forward sequence
|
CACATCCTTCTCACTGCCATGGAATT
|
|
Reverse sequence
|
GCTGCCCTCCCTCCACAGAAAT
|
|
Estrogen Receptor 1
|
RT sequence
|
CCAGGGCCACGCTGGGAAATGAA
|
|
Forward sequence
|
GTTCCAGTGGGCACTGTACTTGGATCTT
|
|
Reverse sequence
|
CAGCTCCATGCCCCAGGGCTAAAT
|
