Epithelial requirement for in vitro proliferation and xenograft growth and metastasis of MDA-MB-468 human breast cancer cells: oncogenic rather than tumor-suppressive role of E-cadherin

Table 2 Antibodies used in this study

	Species	Source	Catalogue number
Primary antibody
Human E-cadherin	Rabbit	Abcam, Cambridge, UK	Ab15148
Human E-cadherin	Mouse	BD Biosciences, San Jose, CA, USA	610404
Human vimentin	Mouse	Dako, Glostrup, Denmark	M0725
α-Pan-actin	Mouse	Abcam, Cambridge, UK	Ab14128
Human mitochondrial	Mouse	Thermo Fisher Scientific, Waltham, MA, USA	MA5-12017
Mouse CD31	Rat	BD Pharmingen, San Diego, CA, USA	550274
Human HIF-1α	Mouse	BD Biosciences, San Jose, CA, USA	610958
Secondary antibody
Antimouse biotin	Rabbit	Dako, Campbellfield, Australia	E0354
Antirabbit biotin	Swine	Dako, Campbellfield, Australia	E0431
Antirat biotin	Goat	Vector Laboratories, Burlingame, CA, USA	BA-9401
Antirabbit Alexa Fluor® 594	Donkey	Molecular Probes, Eugene, OR, USA	R37119
Antimouse Alexa Fluor® 488	Goat	Molecular Probes, Eugene, OR, USA	A11001
Tertiary label
Streptavidin/HRP		Dako, Campbellfield, Australia	P0397

ISSN: 1465-542X