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Fig. 3 | Breast Cancer Research

Fig. 3

From: Epithelial requirement for in vitro proliferation and xenograft growth and metastasis of MDA-MB-468 human breast cancer cells: oncogenic rather than tumor-suppressive role of E-cadherin

Fig. 3

Validation and characterization of E-cadherin-manipulated MDA-MB-468 cell lines. E-cadherin-overexpressing (a) and short hairpin RNA (shRNA)-transduced (b) MDA-MB-468 cells assessed by (i) Western blotting and (ii) quantitative polymerase chain reaction (qPCR) (error bars represent SEM in three independent experiments). Statistical significance was determined by two-tailed, paired t test; *** p < 0.0001, ** p < 0.01 and * p < 0.05. c (i) Treatment of 468-shCDH1 cells with epidermal growth factor (EGF) to assess their ability to undergo epithelial-to-mesenchymal transition (EMT) by qPCR for various EMT genes. Fold expression (from the control) is shown, where control = 1 and y-axis is on a logarithmic scale. Error bars represent SEM derived from three independent experiments. (ii) Matching vimentin immunofluorescence (combined with nuclear 4′,6-diamidino-2-phenylindole [DAPI] stain) of the cells at day 5 time point (scale bar represents 50 μm). ΔCT Cycle threshold change, UTR Untranslated region

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