Fig. 1From: Normal breast tissue DNA methylation differences at regulatory elements are associated with the cancer risk factor ageSubject age is strongly associated with DNA methylation in normal breast tissue independent of cell type. a In the volcano plot, each point represents the associations between DNA methylation and age from cell-type-adjusted multivariable linear models for microarray data (limma) at individual cytosine-guanine dinucleotide (CpG) sites. Increasing -log10 (P value) values on the y-axis show increasing statistical significance and limma effect size on the x-axis positioned away from the zero value reveal the largest DNA methylation changes with age. Significant CpG sites are indicated in red (Q value < 0.01). The gene and gene regions are presented for the five CpG sites with the greatest significance. b Unsupervised clustering of DNA methylation values at age-related CpG sites (Komen, n = 100) visualized alongside CpGs measured in specific cell-types form the Roadmap to Epigenomics data set (n = 691 CpG sites). Each column represents a given tissue sample and each CpG is presented in rowsBack to article page