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Fig. 5 | Breast Cancer Research

Fig. 5

From: Ex vivo expanded natural killer cells from breast cancer patients and healthy donors are highly cytotoxic against breast cancer cell lines and patient-derived tumours

Fig. 5

Restoration of natural killer (NK) cell function following chemotherapy. (1) At time of diagnosis, blood will be collected from the breast cancer patient and peripheral blood mononuclear cells (PBMCs) will be isolated and cryopreserved. (2) Four to five weeks prior to surgery, PBMCs will be thawed and ex vivo expansion will begin via co-culture with K562mbIL-21 aAPCs. (3) Two to three days post-chemotherapy, ex vivo expanded NK cells will be infused back into the patient at a dose of ≥1 × 107/kg body weight. This infusion will be repeated 2 to 3 days after each round of chemotherapy at the same time point as monoclonal antibody (mAb) treatment such as anti-HER2 or anti-GD2 is delivered to the patient. After the last round of chemotherapy and NK cell infusion, (4) mastectomy would be performed followed by (5) radiation therapy

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Breast Cancer Research

ISSN: 1465-542X

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