Fig. 2

Transgenerational effect of maternal control (CON) or high-fat (HF) diet on offspring mammary tumorigenesis. Differences in mammary tumor incidence in (a) F1 (p < 0.016; CON, n = 30 mice; HF, n = 28 mice) and (b) F3 (p < 0.040; CON, n = 19 mice; HF, n = 24 mice) generation female offspring of dams fed either CON or HF diet during pregnancy. Differences in mammary tumor burden in (c) F1 (p < 0.027) and (d) F3 (p < 0.242) generation female offspring. Differences in mammary tumor latency in (e) F1 (p < 0.028) and (f) F3 (p < 0.110) generation female offspring. Mean ± SEM data are shown in c–f. TEB Terminal end bud