Fig. 8

Anchorage-independent growth and epithelial-mesenchymal transition marker expression regulated by myocardin-related transcription factor (MRTF). a MRTF-overexpressing cells were grown embedded in agarose-matrix. Bright field micrographs were taken after 6 weeks. Scale bar 400 μm. b Integrin α5 and α6 staining of 14-day-old acini formed by the indicated cell lines. Scale bar 20 μm. c Protein expression of integrin α5, E-cadherin, vimentin and Snai2/Slug in 2D cultures of MRTF-overexpressing cell lines. Representative immunoblot (left) and quantification of normalized amount of protein (right) is shown. Error bars SEM (n = 3): *p < 0.05, **p < 0.01 (Student’s t test). GAPDH glyceraldehyde-3-phosphate dehydrogenase