Fig. 5

Knockdown of myocardin-related transcription factor (MRTF)-A impairs proliferation and cell cycle. a Knockdown and recue cell lines were cultured in matrigel for 4 days and stained for the proliferation marker (scale bar 20 μm). Quantification of Ki67-positive cells per acini, determined from 30 acini in three different experiments. b, c Proteins from acini recovered at day 14 were immunoblotted for p21 and p27 (b) or phospho-retinoblastoma protein (P-Rb) and retinoblastoma protein (Rb) (c). Upper panels show normalized protein content, and a representative immunoblot is shown below. Error bars SEM (n = 3): *p < 0.05, **p < 0.01 (Student’s t test). SRF serum response factor. shCrtl shRNA control, GAPDH glyceraldehyde-3-phosphate dehydrogenase, GFP green fluorescent protein