Fig. 4

Quantification of acini size, luminal filling and is myocardin-related transcription factor (MRTF) activity. a Quantification of acini size on bright field images. The size of at least 100 acini was measured, each from three independent experiments as described. The box represents the interquartile range and the middle line represents the median; the whiskers extend to the 5th and 95th percentiles. b Quantification of lumen formation detected from confocal images of 30 acini each from three different experiments. c Relative protein expression of MRTF-A and MRTF-B in the knockdown and recue cell lines indicated. Shown is the mean of three independent experiments. A representative immunoblot of MRTF-A and MRTF-B from 2D cell cultures is shown below. d Indicated cell lines were transiently transfected under serum-starved conditions, treated after 24 h, as indicated, and analyzed for MRTF/serum response factor activity. Error bars SEM (n = 3): *p < 0.05, ***p < 0.001 (Student’s t test). shCrtl shRNA control, GAPDH glyceraldehyde-3-phosphate dehydrogenase, GFP green fluorescent protein