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Fig. 3 | Breast Cancer Research

Fig. 3

From: Tightly controlled MRTF-A activity regulates epithelial differentiation during formation of mammary acini

Fig. 3

MCF10A acini formation is myocardin-related transcription factor (MRTF) dependent. Stably transduced cells harboring control or MRTF-A knockdown (shCtrl, shMRTF-A#1, shMRTF-A#2), recue of MRTF-A (sh#1 + MRTF-A, sh#2 + MRTF-A), rescue with MRTF-B (sh#1 + MRTF-B, sh#2 + MRTFB) or control rescue (shCtrl + GFP) were cultured in matrigel for 14 days as before. Immunofluorenscence staining of MRTF-A, MRTF-B, lamininV (red), phalloidin (red) and DNA (blue) from representative confocal mid plane sections are shown. Scale bar 20 μm

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