Fig. 5

Levels of tumor-derived exosomal miR-200c and miR-141 in breast cancer cells and animals. FOXP3 protein expression was measured by western blotting in FOXP3/GFP-Tet-off MCF7 cells. -Dox, doxycycline removed from the culture medium. a-c Quantification of miR-200c and miR-141 (by quantitative (q)PCR) as a percentage of RNU6B or cel-mir-39 expression in FOXP3/GFP-Tet-off MCF7 cells without Dox at 0, 1, 2, 3, and 5 days. Data are presented as means ± SD. *p < 0.05 vs. 0-h group (one-way analysis of variance (ANOVA) followed by the protected least significant difference test). d Levels of miR-200c and miR-141 in mouse blood cells (measured by qPCR) as a percentages of snoRNA202 expression. e Quantification of levels of exosomal miR-200c and miR-141 in mouse plasma (by qPCR) as percentages of cel-mir-39 expression. WT wild-type, Tu tumor, Meta metastasis. *p < 0.05 vs. no tumor group (one-way ANOVA followed by the protected least significant difference test). All experiments were repeated three times