Fig. 4

Plasma levels of miR-200c and miR-141 are elevated in patients with metastatic breast cancer. a Plasma levels of miR-200c and miR-141 presented as adjusted PCR cycle threshold (aCt) values for normal female controls, patients with benign breast tumors, those with a family history of breast cancer, patients with breast ductal carcinoma in situ (DCIS), and patients with breast cancer. The aCt value of each miR was normalized to cel-miR-39 as described previously [4, 21]. In plasma levels of miR-141, *p < 0.05 breast cancer vs. other groups (one-way analysis of variance (ANOVA) followed by the protected least significant difference test). b Plasma miR-200c and miR-141 levels represented as aCt values for patients with localized breast cancer, patients with metastatic breast cancer, and normal female controls. *p < 0.05 disease vs. control group (one-way ANOVA followed by the protected least significant difference test). c, d Sensitivity and specificity of plasma miR-200c and miR-141 for patients with localized breast cancer relative to normal female controls and patients with metastatic breast cancer relative to patients with localized breast cancer. Receiver operating characteristic curves are shown for miR-200c (dotted line) and miR-141 (solid line). AUC area under the curve. e Llevels of miR-200c and miR-141 in blood cells presented as percentages of RUN6B expression for patients with localized breast cancer, patients with metastatic breast cancer, and normal female controls. f Plasma miR-200c and miR-141 levels presented as aCt values for breast cancer patients with estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) status. Data for all patients in this figure are not included in the The Cancer Genome Atlas datasets. All experiments were repeated three times