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Fig. 1 | Breast Cancer Research

Fig. 1

From: MicroRNA-200c and microRNA- 141 are regulated by a FOXP3-KAT2B axis and associated with tumor metastasis in breast cancer

Fig. 1

FOXP3 induces miR-200c and miR-141 in breast cancer cells. a FOXP3 protein expression in green fluorescent protein (GFP) and FOXP3/GFP-Tet-off MCF7 cells was measured by western blotting. -Dox doxycycline removed from the culture medium. b Relative quantification (by quantitative (q)PCR) of miR-200 clusters 1 and 2 in FOXP3/GFP-Tet-off MCF7 cells without Dox at 0, 24, and 48 h. The concentrations of miR-200 s at 0 h were used as a reference. Chr chromosome. Data are presented as means ± SD. *p < 0.05 vs. 0-h group, analysis of variance followed by the protected least significant difference test for differences between groups. c Quantification of miR-200 s (by qPCR) as a percentage of RUN6B expression in T47D (left), BT474 (middle), and MDA-MB-468 (right) cells at 48 h after transfection. Representative western blot analyses for FOXP3 expression in FOXP3-V5-transfected cells (inset in each panel). Data are presented as the means ± SD. *p < 0.05 vs. vector group, two-tailed t test. All experiments were repeated three times

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