Fig. 4

Restoring follistatin (FST) expression abrogates lung metastases in a mouse model of metastatic breast cancer. a Kaplan-Meier plot comparing the percentage of tumor-free mice in a cohort of single transgenic Neu (n = 11) and bitransgenic Neu/FST (n = 8) mice. b Fold change in tumor volume after detection by palpation in single and bitransgenic cohorts. c Representative × 4 magnification images of hematoxylin and eosin-stained lung sections depicting metastases in single and bitransgenic mice. Arrows indicate metastases. d Quantitation of the number of true lung metastases (p < 0.01) and emboli (p < 0.05) in each mouse examined (Neu, n = 10 mice; Neu/FST, n = 8 mice). Statistical significance was determined using Fisher’s exact test