Fig. 5

LincIN interacts with NF90 in the translation regulation of p21 expression. a Left panel: Western blot of p21 and beta-actin in vector and LincIN overexpressed MCF10A cells. Right panel: Western blot of p21, beta-actin or NF90, in SC and LincIN knockdown groups of HeLa cells. b RT-qPCR results of p21 in LincIN overexpression and vector control groups in MCF10A cells (left). RT-qPCR analysis of LincIN knockdown versus scrambled control in HeLa cells (right). (c) and (d) Cell cycle profiles and quantification of MDA-MB-231 cells treated with SC or LincIN siRNAs for 48 h (n = 3). Data was analyzed using one-way ANOVA and t test (^** P < 0.01, and ^* P < 0.05). e Right panel: MCF10A cells (vector or LincIN) were stably transfected with siRNA targeting NF90 or scrambled control. Cell lysates were collected at 48 h after transfection. Left panel: ratios of p21expression in LincIN-overexpression to vector control cells (right panel) (^* P < 0.05; t test). f Right panel: Western blot of NF90, NF45, and p21 in HeLa cells treated with SC, LincIN or NF90 siRNA alone or in combination. Cell lysates were collected at 48 h after transfection, repeated in triplicate. Left panel: ratios of p21expression in siLincIN-transfected to SC control cells (^* P < 0.05; t test)