Fig. 4

LincIN interacts with the NF90/NF45 complex. a Schematic flow of RNA pull-down experiments. b RNA pull-down was performed using the RNA-protein Pull Down Kit. Bands with arrows were submitted for mass spectrometric identification, and the most abundant band was identified as NF90/ILF3. Positive control (PC) and negative control (NC) were HuR and polyA as provided by the RNA pull-down kit, n = 4. c Western blot analysis was used to validate the specific association of NF90 with LincIN in pull-down lysates, repeated experiment. d Left panel: schematic diagram of LincIN fragments; right panel: Western blot analysis of NF90 in eluted protein samples pulled down by in vitro transcribed LincIN fragments. e RT-qPCR analysis of RNP samples (right) enriched by NF90 antibodies. RNP: RNA immunoprecipitation. Data was analyzed using t test (^** P < 0.01, and ^* P < 0.05)