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Fig. 3 | Breast Cancer Research

Fig. 3

From: LincIN, a novel NF90-binding long non-coding RNA, is overexpressed in advanced breast tumors and involved in metastasis

Fig. 3

LincIN mediates breast cancer cell invasion and metastasis in vitro and in vivo. a Inhibitory effects of LincIN knockdown on MDA-MB-231 cell invasion. Left and middle panels: MDA-MB-231 cells were transfected with scrambled control (SC), siLincIN.A or siLincIN.B for 24 hours and seeded on Boyden chambers for in vitro invasion assays. Cell invasion capacity in siLincIN.A-, or siLincIN.B-treated MDA-MB-231 cells was compared to the SC group. Right panel: the RT-qPCR analysis of LincIN knockdown using dicer substrate siLincIN.A or siLincIN.B) versus SC or untreated cells. b Inhibitory effects of LincIN knockdown on HCC1937 cell invasion. c Bioluminescent imaging of mice harboring lung metastases after tail vein injection of MDA-MB-231luc cells stably expressing LincIN shRNAs or empty vector at week 0 and 6. d Bioluminescent quantification plot of lung metastasis by MDA-MB-231luc cells expressing shRNAs or control (empty vector) at day 7, 14, 21, 28, and 43. Data were combined from two independent experiments. e Right panel: representative whole slide imaging (×0.6) of dissected lung tissues from the tail vein injection mice carrying MDA-MB-231luc cells stably expressing LincIN shRNAs or empty vector [stained with hematoxylin and eosin (H&E), left]. Left panel: quantification of lung metastasis was performed using tumor area recorded per lung section against corresponding total lung section area (three mice per group). Data was analyzed using one-way ANOVA and the Dunnett’s test to account for multiple post hoc comparisons (*** P < 0.005, ** P < 0.01, and * P < 0.05; images were taken at ×100). f Top cellular functions targeted by LincIN knockdown by shRNAs (P < 0.01)

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