An international reproducibility study validating quantitative determination of ERBB2, ESR1, PGR, and MKI67 mRNA in breast cancer using MammaTyper®

Table 4 Interclass correlation coefficient based on depicted variance components, and summary of kappa values

Analyte	Intersite variance	Intersample variance	Residual variance	ICC_intra	ICC_inter	kappa Statistic
ERBB2	0.039	2.886	0.054	0.982	0.987	1.00
ESR1	0.049	5.889	0.144	0.976	0.992	0.91
PGR	0.013	8.279	0.031	0.996	0.998	0.94
MKI67	0.032	1.565	0.039	0.976	0.980	0.94
Subtype	N/A					0.90
Analyte	Intersite variance (present study)	Intersample variance (FinHer data)	Residual variance (present study)	ICC_intra	ICC_inter	kappa Statistic (median)	2.5–97.5% percentile
ERBB2	0.039	3.723	0.054	0.986	0.990	0.95	0.92–0.97
ESR1	0.049	5.941	0.144	0.977	0.992	0.97	0.96–0.99
PGR	0.013	7.461	0.031	0.996	0.998	0.97	0.96–0.99
MKI67	0.032	1.739	0.039	0.978	0.982	0.85	0.82–0.88
Subtype	N/A					0.91	0.89-0.93

Abbreviations: ERBB2 (HER2) Human epidermal growth factor receptor 2, ESR1 (ER) Estrogen receptor, MKI67 Marker of proliferation Ki67, PGR (PgR) Progesterone receptor, N/A Not applicable
Note: Intersite variance and residual variance are squares of SD presented in lower part of Table 1. The lower panel of this table is a simulated analysis using the intersample variance of data from the FinHer trial cohort (described in detail in the text)
Boldface values highlight ICC and kappa statistic

ISSN: 1465-542X