An international reproducibility study validating quantitative determination of ERBB2, ESR1, PGR, and MKI67 mRNA in breast cancer using MammaTyper®

Table 2 Binary single-marker agreements as positive/negative counts, and corresponding kappa values (distance of mean 40^−∆∆Cq value to marker-specific cutoff is shown for each sample)

	ERBB2			ESR1			PGR			MKI67
Sample	Counts		Distance to cutoff (C_q)	Counts		Distance to cutoff (C_q)	Counts		Distance to cutoff (C_q)	Counts		Distance to cutoff (C_q)
Sample	Negative	Positive	Distance to cutoff (C_q)	Negative	Positive	Distance to cutoff (C_q)	Negative	Positive	Distance to cutoff (C_q)	Negative	Positive	Distance to cutoff (C_q)
1	40	0	−1.1	0	40	1.5	0	40	3.2	40	0	−3.2
2	40	0	−1.1	0	40	1.7	0	40	1.8	40	0	−0.8
3	0	40	0.6	0	40	1.9	0	40	2.5	0	40	0.8
4	40	0	−1.2	0	40	3.0	0	40	4.6	0	40	0.7
5	40	0	−1.9	0	40	1.6	0	40	5.6	0	40	0.6
6	40	0	−2.9	0	40	1.4	0	40	6.0	0	40	0.6
7	40	0	−3.2	40	0	−4.0	40	0	−3.0	0	40	2.2
8	0	40	1.7	12	28	0.2	0	40	2.9	0	40	1.3
9	0	30	1.3	30	0	−3.2	30	0	−2.3	0	30	1.1
10	0	30	3.1	30	0	−2.0	30	0	−1.7	0	30	1.5
11	30	0	−1.6	0	30	2.1	0	30	4.9	4	26	0.2
12	30	0	−1.8	0	30	2.7	0	30	4.7	0	30	1.1
13	30	0	−1.8	0	30	2.4	0	30	4.6	1	29	0.4
14	30	0	−2.0	0	30	1.4	0	30	3.2	30	0	−0.8
15	30	0	−1.3	0	30	1.1	0	30	1.0	29	1	−1.2
16	29	0	−1.5	0	29	2.5	0	29	2.9	0	29	0.7
17	30	0	−1.1	0	30	1.4	0	30	0.8	0	30	1.1
18	30	0	−1.3	0	30	2.5	0	30	4.2	30	0	−1.2
19	30	0	−1.5	0	30	2.8	0	30	2.8	1	29	0.4
20	30	0	−2.6	30	0	−0.9	0	30	3.3	29	1	−0.6
21	30	0	−2.8	20	10	0.0	0	30	3.4	29	1	−0.5
22	30	0	−3.1	30	0	−3.4	30	0	−3.1	0	30	2.1
23	30	0	−3.1	30	0	−2.3	16	14	0.0	0	30	2.2
24	0	30	1.9	30	0	−5.2	30	0	−3.3	1	29	0.4
kappa Statistic	1.00			0.91			0.94			0.94

Abbreviations: ERBB2 (HER2) Human epidermal growth factor receptor 2, ESR1 (ER) Estrogen receptor, MKI67 Marker of proliferation Ki67, PGR (PgR) Progesterone receptor
Boldface values represent summary of measurements