Fig. 2

Myoepithelial and luminal cells are susceptible to lentiviral infection and re-form into spheroid and ductal structures in collagen-based gels. a Azurite blue expression in myoepithelial and luminal cells 48 h following lentiviral delivery with or without neuraminidase (20 mU/ml) pre-treatment. Scale bar = 20 μm. b Representative bright-field images of isolated myoepithelial and luminal cells grown together for 21 days in collagen- or Matrigel-based gels. Scale bar = 200 μm. c and d Representative confocal images of green fluorescent protein-labelled myoepithelial and azurite blue-labelled luminal cells grown in collagen-based (c) or Matrigel-based (d) gels. Images are representative of cells derived from at least three donors. Scale bar = 20 μm