Fig. 4
From: TIMELESS contributes to the progression of breast cancer through activation of MYC
MYC is the downstream effector molecule of TIM. a Gene set enrichment analysis showing that TIM expression positively correlated with MYC-regulated gene signatures in The Cancer Genome Atlas (TCGA) dataset. b Western blot analysis of protein levels of MYC in MCF-7 and T47D with TIM overexpression or knockdown. c, d Real-time PCR analysis of the mRNA levels of MYC in MCF-7 and T47D with TIM overexpression (c) or knockdown (d). e Luciferase analysis indicating the transactivity of MYC in MCF-7 and T47D with TIM overexpression or knockdown. *P < 0.05. Each bar represents mean ± SD of three independent experiments. f Real-time PCR analysis of the mRNA levels of MYC target genes including CDK4, PKM2, LDHA2 and CAV1 in MCF-7 and T47D with TIM overexpression or knockdown