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Fig. 2 | Breast Cancer Research

Fig. 2

From: TIMELESS contributes to the progression of breast cancer through activation of MYC

Fig. 2

TIM contributes to the expansion of cancer stem cells and cell migration and invasion of breast cancer. a Primary and secondary mammosphere formation analysis of MCF-7 and T47D with TIM overexpression or knockdown (left panel) and quantification of mammosphere number (right panel). Representative photographs of primary mammosphere and mammosphere number (upper). Representative photographs of secondary mammosphere and mammosphere number (bottom). *P < 0.05. b Flow cytometry analysis of side population (SP) cell proportion in the indicated cells. c Flow cytometry analysis of CD24-CD44+ subpopulation in indicated cells. d Wound healing assays showing the migratory abilities of the indicated cells. e Transwell assays showing the invasive ability of the indicated cells (left panel) and quantification of invaded cell number (right panel). *P < 0.05. Each bar represents mean ± SD of three independent experiments

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