Overexpression of syndecan-1, MUC-1, and putative stem cell markers in breast cancer leptomeningeal metastasis: a cerebrospinal fluid flow cytometry study

Table 2 CSF flow cytometry characterization of cancer floating cells in 13 cases of breast cancer leptomeningeal metastasis

Case number	CSF cellularity (cells/μl)	Number of events analyzed	Percentage of BC cells (CD45-negative)	Percentage of positive cells within the CD45-negative BC population
Case number	CSF cellularity (cells/μl)	Number of events analyzed	Percentage of BC cells (CD45-negative)	CD15%	CD138%	CD227%	CD24%	CD44%	CD133%
1	32	181,000	9	nd	82	nd	nd	nd	nd
2	1	2887	47	nd	99	nd	nd	nd	nd
3	86	132,260	43	24	96	nd	98	94	96
4	66	12,817	96	51	97	nd	nd	nd	neg
5	2	6078	4	75	80	nd	nd	nd	93
6	50	45,134	20	neg	91	93	94	97	neg
7	1	4612	20	66	54	97	90	88	59
8	2	1183	35	nd	80	94	nd	87	80
9	30	24,300	47	neg	56	82	neg	neg	neg
10	17	29,798	40	92	98	96	77	96	neg
11	1	1293	60	12	93	96	100	99	neg
12	8	3550	45	98	89	97	neg	56	neg
13	8	8455	36	neg	66	88	89	neg	88

Breast cancer marker expression is reported as the percentage of positive cells within the CD45-negative/side-scatter large population
CSF cerebrospinal fluid, BC breast cancer, nd not done, neg negative

ISSN: 1465-542X